It is commonly recognized that excessive cell growth is the hallmark of cancer ( 4). The increasing incidence and mortality rates of pancreatic cancer have prompted investigations into new drug therapies for the comprehensive treatment of patients with pancreatic cancer. Chemotherapy is also widely used for the treatment of pancreatic cancer in the clinic, but most patients respond poorly to several chemotherapeutic drugs. At present, surgical excision is the best curative treatment for patients with pancreatic cancer, but most patients do not recognize the symptoms in time, especially in the early stages ( 2, 3). The 5-year survival rate of pancreatic cancer patients is nearly 8%, and the survival rate in patients with advanced disease is only 3% ( 1). Accepted for publication Apr 15, 2022.Īs one of the most aggressive and deadliest malignancies, pancreatic cancer seriously threatens human health.
Keywords: Bee venom pancreatic cancer cell proliferation apoptosis migration Experimental data also showed that bee venom induced cell apoptosis and impeded cell migration.Ĭonclusions: The present study revealed that bee venom could effectively inhibit tumor progression in pancreatic cancer cells, indicating the possibility of bee venom as an anti-tumor drug in pancreatic cancer. At the same time, bee venom can activate the p53–p21 pathway. Bee venom induced S phase arrest and ameliorated the protein expression of cyclins and cyclin-dependent kinases (CDKs). Results: Results demonstrated that bee venom significantly suppressed cell proliferation via inducing cell cycle arrest and apoptosis with suppression of cell migration. Western blot was performed to assess the expression of proteins involved in the regulation of cell cycle arrest and apoptosis. In addition, cell migration was analyzed by the cell scratch test and Transwell assay.
Then, we evaluated the cell cycle and apoptosis by flow cytometry and the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. Methods: Firstly, the Cell Counting Kit-8 (CCK-8) assay was conducted to analyze the cytotoxicity of bee venom on PANC-1 and AsPC-1 cells. However, little is known about the effect of bee venom on pancreatic cancer. Due to its biological activity, bee venom is widely used as an anti-inflammatory agent and pain reliever. Bee venom is a mixture of enzymes, peptides, and amines.
Policy of Dealing with Allegations of Research Misconductīackground: Pancreatic cancer seriously threatens human health.Policy of Screening for Plagiarism Process.
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